Antigen Information

Gene Symbols:

ABL1 | AXL | BLK | BMX | BTK | CSF1R | CSK | DDR2 | EGFR | EPHA1 View more

Assay Format

Pathway:

PI3K-AKT Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ABL1	ACK	ALK-1	Axl	Blk
BMX	Btk	Csk	Dtk	EGFR
EphA1	EphA2	EphA3	EphA4	EphA5
EphA6	EphA7	EphA8	EphB1	EphB2
EphB3	EphB4	EphB6	ErbB2	ErbB3
ErbB4	FAK	FER	FGFR1	FGFR2
FGFR2 alpha isoform	Fgr	FRK	Fyn	Hck
HGFR	IGF-1 R	Insulin R (CD220)	Itk	JAK1
JAK2	JAK3	LCK	LTK	Lyn
MATK	M-CSF R	MUSK	NGFR (TNFRSF16)	PDGFRA
PDGFRB	PYK2	RET	ROR1	ROR2
ROS	RYK	SCF R (CD117/c-kit)	SRMS	SYK
Tec	Tie-1	Tie-2	TNK1	TRKB
TXK	Tyk2	TYRO10 (DDR2/TKT)	VEGFR2	VEGFR3
ZAP70

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human RTK Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Anti-Phospho Tyrosine Detection Antibody
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer (1 x 5 ml)
Protease Inhibitor Cocktail
Phosphatase Inhibitor Cocktail II
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.