RayBio® C-Series Human Insulin Receptor Pathway Phosphorylation Array 1 Kit. Detects 9 phosphorylated proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.


 AAH-INSR-1-2

Antigen Information

Gene Symbols:

  • EIF4E, FOXO3, IGF1R, INPP5D, INS, IRS1, PTPN11, SHC1, STK11

Assay Format

Pathway:

  • Akt Signaling

  • IGF Signaling

  • mTOR Signaling

  • PI3K-AKT Signaling

Number of Targets Detected:
9
Species Detected:

  • Human

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use

  • No specialized equipment needed

  • Compatible with nearly any liquid sample

  • Proven technology (many publications)

  • Highly sensitive (pg/ml)

  • Sandwich ELISA specificity

  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

eIF-4E (P-Ser209)
FOXO-3 (P-Ser413)
IGF-1 R (P-Tyr1165)
Insulin (P-Tyr1189)
IRS-1 (P-Ser318)
LKB1 (P-Ser428)
SHC (P-Tyr427)
SHIP1 (P-Tyr1020)
SHP-2 (P-Ser542)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components

  • Human Insulin Receptor Phosphorylation Array C1 Membranes

  • Blocking Buffer

  • Detection Antibody Cocktail

  • 1,000X HRP-Anti-Rabbit IgG Concentrate

  • 20X Wash Buffer I Concentrate

  • 20X Wash Buffer II Concentrate

  • 2X Cell Lysis Buffer Concentrate

  • Detection Buffer C

  • Detection Buffer D

  • 8-Well Incubation Tray w/ Lid

  • Protease Inhibitor Cocktail

  • 100x Phosphatase Inhibitor Cocktail I

  • Phosphatase Inhibitor Cocktail II

  • Plastic Sheets

  • Array Map Template

  • Manual

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Tissue Paper, blotting paper or chromatography paper

  • Adhesive tape or Saran Wrap

  • Distilled or de-ionized water

  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

  1. Block membranes

  2. Incubate with Sample

  3. Incubate with Detection Antibody Cocktail

  4. Incubate with HRP-Conjugated anti-IgG

  5. Incubate with Detection Buffers

  6. Image with chemiluminescent imaging system

  7. Perform densitometry and analysis

Typical Data

Figure 1 - HepG2 or HeLa cells were grown to 80% confluency and then serum starved overnight. HepG2 Cells were treated with IGF for 10 minutes. HeLa Cells were treated with Calyculin A for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Human Insulin Receptor Pathway Phosphorylation Array C1(图1)

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.