Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
bad	bax	bcl-2	bcl-w	BID
BIM	Caspase-3	Caspase-8	CD40 (TNFRSF5)	CD40 Ligand (TNFSF5)
cIAP-2	Cytochrome C	DR6 (TNFRSF21)	Fas (TNFRSF6/Apo-1)	Fas Ligand (TNFSF6)
HSP27	HSP60	HSP70	HTRA2	IGF-1
IGF-2	IGFBP-1	IGFBP-2	IGFBP-3	IGFBP-4
IGFBP-5	IGFBP-6	IGF-1 R	livin	p21
p27	p53	SMAC	Survivin (BIRC5)	TNF RI (TNFRSF1A)
TNF RII (TNFRSF1B)	TNF alpha	TNF beta (TNFSF1B)	TRAIL R1 (TNFRSF10A/DR4)	TRAIL R2 (TNFRSF10B/DR5)
TRAIL R3 (TNFRSF10C)	TRAIL R4 (TNFRSF10D)	XIAP

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Antibody Array G1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Protease Inhibitor Cocktail
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.