Antigen Information

Gene Symbols:

Assay Format

Pathway:

Akt Signaling
IGF Signaling
p53 Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad	bax	bcl-2	bcl-w	BID
BIM	Caspase-3	Caspase-8	CD40 (TNFRSF5)	CD40 Ligand (TNFSF5)
cIAP-2	Cytochrome C	DR6 (TNFRSF21)	Fas (TNFRSF6/Apo-1)	Fas Ligand (TNFSF6)
HSP27	HSP60	HSP70	HTRA2	IGF-1
IGF-2	IGFBP-1	IGFBP-2	IGFBP-3	IGFBP-4
IGFBP-5	IGFBP-6	IGF-1 R	livin	p21
p27	p53	SMAC	Survivin (BIRC5)	TNF RI (TNFRSF1A)
TNF RII (TNFRSF1B)	TNF alpha	TNF beta (TNFSF1B)	TRAIL R1 (TNFRSF10A/DR4)	TRAIL R2 (TNFRSF10B/DR5)
TRAIL R3 (TNFRSF10C)	TRAIL R4 (TNFRSF10D)	XIAP

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
Protease Inhibitor Cocktail
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.