RayBio® G-Series Mouse Inflammation Antibody Array 1 Kit. Detects 40 Mouse Inflammatory Factors. Suitable for all liquid sample types.


 AAM-INF-G1-4

Antigen Information

Gene Symbols:
CCL1 | CCL11 | CCL2 | CCL24 | CCL25 | CCL3 | CCL5 | CCL9 | CSF1 | CSF2   View more

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Mouse

Compatible Sample Types:
  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:
  • Sandwich-based

Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity

  • Low sample volume (10-100 µl per array)

  • Large dynamic range of detection

  • Compatible with most sample types

  • Test 4 or 8 samples on each slide

  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
BLC
(CXCL13)
CD30 Ligand
(TNFSF8)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Fas Ligand
(TNFSF6)
Fractalkine
(CX3CL1)
GCSF
GM-CSF
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-6
IL-9
IL-10
IL-12 p40/p70
IL-12 p70
IL-13
IL-17A
I-TAC
(CXCL11)
KC
(CXCL1)
Leptin
LIX
Lymphotactin
(XCL1)
MCP-1
(CCL2)
M-CSF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
RANTES
(CCL5)
SDF-1 alpha
(CXCL12 alpha)
I-309
(TCA-3/CCL1)
TECK
(CCL25)
TIMP-1
TIMP-2
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Mouse Inflammation Antibody Array G1 Slide(s)

  • Blocking Buffer

  • Wash Buffer 1

  • Wash Buffer 2

  • Biotinylated Detection Antibody Cocktail

  • Streptavidin-Conjugated Fluor

  • Lysis Buffer

  • Adhesive Plastic Strips

  • 30 ml Centrifuge Tube

  • Manual

Other Materials Required
  • Small plastic boxes or containers

  • Pipettors, pipette tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Aluminum foil

  • Gene microarray scanner or similar laser fluorescence scanner


View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide

  2. Block array surface

  3. Incubate with Sample

  4. Incubate with Biotinylated Detection Antibody Cocktail

  5. Incubate with Streptavidin-Conjugated Fluor

  6. Disassemble the glass slide

  7. Scan with a gene microarray laser scanner

  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.