Gene Symbols:CCL1 | CCL11 | CCL15 | CCL2 | CCL24 | CCL3 | CCL4 | CCL5 | CCL8 | CSF1
View more Number of Targets Detected:40
Method of Detection:Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Glass Slide
High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays
| Scroll over each target protein for more information |
|---|
Eotaxin-1
(CCL11) | Eotaxin-2
(MPIF-2/CCL24) | GCSF | GM-CSF | ICAM-1
(CD54) |
IFN-gamma | I-309
(TCA-3/CCL1) | IL-1 alpha
(IL-1 F1) | IL-1 beta
(IL-1 F2) | IL-2 |
IL-3 | IL-4 | IL-6 | IL-6 R | IL-7 |
IL-8
(CXCL8) | IL-10 | IL-11 | IL-12 p40 | IL-12 p70 |
IL-13 | IL-15 | IL-16 | IL-17A | IP-10
(CXCL10) |
MCP-1
(CCL2) | MCP-2
(CCL8) | M-CSF | MIG
(CXCL9) | MIP-1 alpha
(CCL3) |
MIP-1 beta
(CCL4) | MIP-1 delta
(CCL15) | RANTES
(CCL5) | TGF beta 1 | TNF alpha |
TNF beta
(TNFSF1B) | TNF RI
(TNFRSF1A) | TNF RII
(TNFRSF1B) | PDGF-BB | TIMP-2 |
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
Human Inflammation Antibody Array G3 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual
Other Materials Required
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.