RayBio® L-Series Human Antibody Array 2000 Glass Slide Kit. Detects 2000 Human Proteins. Suitable for serum, plasma and cell culture supernatants. (4 or 8 Sample Kit)


 AAH-BLG-2000-4

Antigen Information

Gene Symbols:
A1BG | A2M | ABAT | ABCC1 | ABCF1 | ABI3BP | ABL1 | ACAA1 | ACAA2 | ACACA   View more

Assay Format

Number of Targets Detected:
2000
Species Detected:

  • Human

Compatible Sample Types:

  • Cell Culture Supernatants

  • Plasma

  • Serum

Design Principle:

  • Label-based

Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High density arrays

  • 2000 Human proteins

  • Direct biotin labeling of proteins

  • High detection sensitivity

  • Accurate and reproducible

  • Affordable, quick and simple to use

Target Names

11b-HSD1, 14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 2B4, 2Mannosidase IA, 4-1BB, 53BP1, 6Ckine, A1BG, A2M, ABAT, ABCF1, ABI3BP, ABL1, ACAA, ACAA1, ACAA2, ACACA, ACE, ACE-2...View all 2000 human target proteins

Target Species

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components

  • RayBio® L-Series Human L2000 Antibody Array Glass Slide(s)

  • Dialysis Vials & Floating Dialysis Rack

  • Labeling Reagent

  • Stop Solution

  • Blocking Buffer

  • 20x Wash Buffer 1

  • 20x Wash Buffer 2

  • Cy3-Conjugated Streptavidin

  • Adhesive Plastic Strips

  • Labeling Buffer

  • 2z Cell Lysis Buffer

  • 30 ml Centrifuge Tube

Other Materials Required

  • Distilled or de-ionized water

  • KCl, NaCl, KH2PO4, Na2HPO4, and ddH20

  • 1 ml tube, small plastic or glass containers

  • Orbital shaker or oscillating rocker

  • Beaker, stir plate and stir bar

  • Pipettors, pipette tips and other common lab consumables

  • Laser scanner for fluorescence detection

  • Aluminum foil


View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline

  1. Dialyze samples in PBS

  2. Biotinylate Samples

  3. Dialyze biotinylated samples using spin column

  4. Dry the glass slide

  5. Block array surface

  6. Incubate with Samples

  7. Incubate with Streptavidin-Conjugated Fluor

  8. Disassemble the glass slide

  9. Scan with a gene microarray laser scanner

  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.