More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 200 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation
4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ALCAM (CD166), Amphiregulin, AXL, BCMA, BDNF, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-7, CCL28 (MEC), CD14, CD30 (TNFRSF8), CD40 Ligand (TNFSF5), CD80 (B7-1), CEACAM-1, Contactin-2 (TAG1), CTACK (CCL27), CXCL16, DR6 (TNFRSF21), Dtk, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB3, E-Selectin, Fas (TNFRSF6/Apo-1), FGF-4, FGF-7 (KGF), Flt-3 Ligand, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GM-CSF, GRO alpha/beta/gamma, Growth Hormone, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-3 (CD50), IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17 RA, IL-17A, IL-17F, IL-18 BP alpha, IL-2, IL-2 R gamma (Common gamma Chain), IL-21 R, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-31, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF R beta, PDGF-AA, PDGF-BB, PECAM-1, Platelet Factor 4 (CXCL4), PLGF, RAGE, RANTES (CCL5), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TECK (CCL25), TGF alpha, TGF beta 1, TGF beta 3, TIM-1 (KIM-1), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R3 (TNFRSF10C), Trappin-2, TSLP, uPAR, VCAM-1 (CD106), VEGF-A, VEGF-D, VEGFR2, VEGFR3, XEDAR
Suggested Applications
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
Human Cytokine Array Q3000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual
Other Materials Required
Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.