Human L3 Array, Membrane

    


RayBio® L-Series Human Antibody Array 3 Membrane Kit. Detects 500 Human Proteins. Suitable for cell culture supernatants (A) and serum, plasma and tissue lysates (B).



 AAH-BLM-3A-2

Antigen Information

Gene Symbols:
ABAT | ABCF1 | ABI3BP | ACAA1 | ACAA2 | ACACA | ACAN | ACAT2 | ACLY | ACO1   View more

Assay Format

Number of Targets Detected:
500
Species Detected:
  • Human

Compatible Sample Types:
  • Cell Culture Supernatants

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:
  • Label-based

Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2 or 4 Sample Kit

Product Features

  • High density arrays

  • 500 Human proteins

  • Direct biotin labeling of proteins

  • High detection sensitivity

  • Accurate and reproducible

  • Affordable, quick and simple to use

Target Names

14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, LAMR1, ABAT, ABCF1, ABI3BP, ACAA1, ACAA2, ACACA, ACAA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAMDEC1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK , Ahsp, AIF, AK2 , AKAP9 , AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1 , ALDH1A1, ALDH9A1, Alkaline Phosphatase, ALP, alpha 1,2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha-synuclein, alpha Tubulin, AlphaA Crystallin, ALS, Als2 , ALS+B62:B1212CR1 , Aminoacylase-1, Androgen Receptor, ANGPTL6, ANGPTL8, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, Annexin V, ANP, Antithrombin 3, APA, APLP-1, APM2, Apo (a), APOA1BP, ApoF, ApoL1, ApoL2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, Aminopeptidase PILS, ARX, ASH2L , Asialoglycoprotein Receptor 2, ASK1 , Aspartate Aminotransferase , Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, B7-H2, B7-H3, bad, Band 3, BASP1, Bassoon, BAZ2B, BCHE, bcl-w, BCOR, beta 1 Spectrin , beta B1 Crystallin, Tubulin beta-1 chain, Tubulin beta-3 chain, BID, BIN2, BIRC6, BLMH, BLVRB, BMP-1, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C1S, C3orf75, C4.4A, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150 , CA2, CA3, CACNB4, CAD , Cadherin-22, Cadherin-6, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1 , Calpastatin, Calretinin, Calumenin , CAP1, CapG, CAPZA1, Carboxypeptidase B2, CARHSP1, Caspase-14, Catalase, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, Cathepsin Z, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD155, ADP-Ribosyl Cyclase 2, CD16, CD21, CD32, CD35, CD39L4, CD41, CD42b, CD48, CD5L, CD9 , CD98, CDA, CDC5L, CDK2 , CEACAM-8, CECR1, CENPF, CEP57, Carboxylesterase 1, CETP, Cezanne, CFHR 1, CFHR4, Complement Factor H-related 5, CFI, CFL1, Coagulation Factor VII, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, Collectin-12, CLPS, CLTA, CNN2, CNOT1, CO4A2, COG4 , COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Thrombospondin 5, Complement Factor B, Contactin-3, COPS8 , Corneodesmosin, Coronin 3, Cortactin, COTL1, Carboxypeptidase E, CPEB3, Carboxypeptidase M, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin A, Cyclophilin B, Cystatin D, Cystatin E/M, Cystatin S, Cystatin SN, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytochrome C, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 20, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DAN, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, DEP-1, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin , DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, Ubiquitin-activating enzyme E1, ECHS1 , ECM-1, EEF1G , EEF2, EFEMP2 , EFTUD2, EHD1 , EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1 , EMILIN1 , EMSY, EN2, Endorepellin, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, Aminopeptidase LRAP, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, Coagulation Factor XI, FABP5, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, F-box protein 38, FBPase 2, FBPase 1, FCGBP, FDPS , FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FSH, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP , GALNT2, Catenin gamma, GAPHD, GARNL1 , GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC , GCSH , GDA, GDF7, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, G6PD, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma , GSTO1, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, GPV, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLM1, GOLPH4 , GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GRP, GSTM1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, Growth Hormone, hHR23b, HIBADH , HINT1, HIP1R , Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha , hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G , hnRNP L, hnRNP M1-M4 , hnRNP U , Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRT, HPRG, HRSP12, HSC70, HSP47

Target Species

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human 3 Antibody Array Membranes

  • Labeling Reagent

  • Stop Solution

  • Blocking Buffer

  • HRP-Conjugated Streptavidin Concentrate

  • Detection Buffer C

  • Detection Buffer D

  • Plastic Sheets

  • Floating Dialysis Rack

  • Dialysis Vials

  • Wash Buffer 1

  • Wash Buffer 2

  • Spin Columns

  • Plastic Incubation Tray

  • (Lysate specific kit) Lysis Buffer

Other Materials Required
  • Distilled or de-ionized water

  • KCl, NaCl, KH2PO4 and Na2HPO4

  • Small plastic or glass containers

  • Orbital shaker or oscillating rocker

  • Beaker, stir plate and stir bar

  • 1 ml tube

  • Pipettors, pipette tips and other common lab consumables

  • Laser scanner for fluorescence detection (list of compatible scanners available at http://www.raybiotech.com/resources.asp)

  • Aluminum foil

Protocol Outline
  1. Dialyze samples in PBS

  2. Biotinylate Samples

  3. Dialyze biotinylated Samples in PBS

  4. Block array membranes

  5. Incubate with Samples

  6. Incubate with HRP-Conjugated Streptavidin

  7. Incubate with Detection Buffers

  8. Image with chemiluminescent imaging system

  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.