Human Cytokine Array C1000

RayBio® C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.

CODE: AAH-CYT-1000-2

Number of Membranes:

Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

120

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin (ACRP30)	AgRP	Amphiregulin	Angiogenin	Angiopoietin-2
Axl	BDNF	bFGF	BLC (CXCL13)	BMP-4
BMP-6	beta-NGF	Betacellulin (BTC)	CCL28 (MEC)	CK beta 8-1 (CCL23)
CNTF	CTACK (CCL27)	Dtk	EGF	EGFR
ENA-78 (CXCL5)	Eotaxin-1 (CCL11)	Eotaxin-2 (MPIF-2/CCL24)	Eotaxin-3 (CCL26)	Fas (TNFRSF6/Apo-1)
FGF-4	FGF-6	FGF-7 (KGF)	FGF-9	Flt-3 Ligand
Fractalkine (CX3CL1)	GCP-2 (CXCL6)	GCSF	GDNF	GITR (TNFRSF18)
GITR Ligand (TNFSF18)	GM-CSF	GRO alpha/beta/gamma	GRO alpha (CXCL1)	HCC-4 (CCL16)
HGF	I-309 (TCA-3/CCL1)	ICAM-1 (CD54)	ICAM-3 (CD50)	IFN-gamma
IGFBP-1	IGFBP-2	IGFBP-3	IGFBP-4	IGFBP-6
IGF-1	IGF-1 R	IL-1 R4 (ST2)	IL-1 R1	IL-10
IL-11	IL-12 p40	IL-12 p70	IL-13	IL-15
IL-16	IL-17A	IL-1 alpha (IL-1 F1)	IL-1 beta (IL-1 F2)	IL-1 ra (IL-1 F3)
IL-2	IL-2 R alpha	IL-3	IL-4	IL-5
IL-6	IL-6 R	IL-7	IL-8 (CXCL8)	I-TAC (CXCL11)
Leptin	Light (TNFSF14)	Lymphotactin (XCL1)	MCP-1 (CCL2)	MCP-2 (CCL8)
MCP-3 (MARC/CCL7)	MCP-4 (CCL13)	M-CSF	MDC (CCL22)	MIF
MIG (CXCL9)	MIP-1 alpha (CCL3)	MIP-1 beta (CCL4)	MIP-1 delta (CCL15)	MIP-3 alpha (CCL20)
MIP-3 beta (CCL19)	MSP alpha/beta	NAP-2 (PPBP/CXCL7)	NT-3	NT-4
Oncostatin M	Osteoprotegerin (TNFRSF11B)	PARC (CCL18)	PDGF-BB	PLGF
RANTES (CCL5)	SCF	SDF-1 alpha (CXCL12 alpha)	gp130	TNF RII (TNFRSF1B)
TNF RI (TNFRSF1A)	TARC (CCL17)	TECK (CCL25)	TGF beta 3	TGF beta 1
Thrombopoietin (TPO)	TIMP-1	TIMP-2	TNF alpha	TNF beta (TNFSF1B)
TRAIL R3 (TNFRSF10C)	TRAIL R4 (TNFRSF10D)	uPAR	VEGF-A	VEGF-D

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C1000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.