Rapid Mouse Ig Isotyping Array 1

    


RayBio® Rapid Mouse Immunoglobulin Isotyping Array 1. Detects 8 Mouse Ig sub-classes and 2 light chain types. Suitable for serum, plasma, and cell culture supernatants.



 AAM-ISO-G1-1

Antigen Information

Gene Symbols:

  • IGA

  • IGE

  • IGG2A

  • IGHG1

  • IGM

Assay Format

Number of Targets Detected:
10
Species Detected:

  • Mouse

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16, 32, or 64 Sample Kit

Product Feature

  • One step mouse monoclonal antibody isotyping

  • Use only 1-2 µl sample

  • The whole experiment can be done within 1 hour

  • Sandwich based technology for high specificity and sensitivity

  • Low system CV with high reproducibility

  • High throughput sample processing

  • Qualitative visual inspection or semi-quantitative result

  • Processed slides can be stored for years without signal decay

Target Name

IgA, IgD, IgE, IgM, IgG1, IgG2a, IgG2b, and IgG3 Heavy Chains; Kappa and Lambda Light Chains

Target Species

Mouse

Application Notes

Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components

  • Mouse Ig Rapid Isotyping Glass Slide(s)

  • Sample Diluent

  • 20X Wash Buffer

  • Labeled Detection Antibody

  • Slide Washer/Dryer

  • 96-well Plate

  • Manual

Other Materials Required

  • Small plastic boxes or containers

  • Pipettors, pipette tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Aluminum foil

  • Gene microarray scanner or similar laser fluorescence scanner

Protocol Outline

  1. Dry the glass slide

  2. Mix samples with detection antibodies (in 96-well plate)

  3. Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)

  4. Disassemble the glass slide

  5. Scan with a gene microarray laser scanner

  6. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Quantibody Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.