Rapid Rat Ig Isotyping Array 1

    


RayBio® Rapid Rat Immunoglobulin Isotyping Array 1. Detects 6 Rat Ig sub-classes and 2 light chain types. Suitable for serum, plasma, and cell culture supernatants.



 AAR-ISO-G1-1

Antigen Information

Gene Symbols:

  • IGA

  • IGG2A

  • IGG2B

  • IGHG1

  • IGM

Assay Format

Number of Targets Detected:
8
Species Detected:

  • Rat

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16, 32, or 64 Sample Kit

Product Feature

  • One step rat monoclonal antibody isotyping

  • Use only 1-2 µl sample

  • The whole experiment can be done within 1 hour

  • Sandwich based technology for high specificity and sensitivity

  • Low system CV with high reproducibility

  • High throughput sample processing

  • Qualitative visual inspection or semi-quantitative result

  • Processed slides can be stored for years without signal decay

Target Name

Heavy Chains: IgA, IgM, IgG1, IgG2a, IgG2b, IgG2c
Light Chains: Kappa, Lambda

Application Notes

Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components

  • Rat Ig Rapid Isotyping Array Glass Slide(s)

  • Sample Diluent

  • 20X Wash Buffer

  • Labeled Detection Antibody

  • Slide Washer/Dryer

  • 96-well Plate

  • Manual

Other Materials Required

  • Small plastic boxes or containers

  • Pipettors, pipette tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Aluminum foil

  • Gene microarray scanner or similar laser fluorescence scanner


View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline

  1. Dry the glass slide

  2. Mix samples with detection antibodies (in 96-well plate)

  3. Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)

  4. Disassemble the glass slide

  5. Scan with a gene microarray laser scanner

  6. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Isotyping Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.