High density arrays
90 Rat proteins
Direct biotin labeling of proteins
High detection sensitivity
Accurate and reproducible
Affordable, quick and simple to use
Activin A, ACTH, ADFP, Adiponectin, AMPK alpha 1, B7-1/CD80, BDNF, beta-Catenin, bFGF, b-NGF, CCR4, CD106, CINC-2 alpha/beta, CINC-3, CNTF, CNTF R alpha, CSK, CXCR4, EGFR, EG-VEGF/PK1, E-Selectin, FADD, Fas Ligand/TNFSF6, Fas/TNFRSF6, FGF-BP, Follostatin-like -1 (FSL1), Fractalkine, GFR alpha-1, GFR alpha-2, GM-CSF, Growth Hormone, Growth Hormone R, Hepassocin, ICAM-1/CD54, ICK, Insulin Degrading Enzyme (IDE), IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 R6/IL-1 R rp2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23 p40, IL-13, Integrin alpha M beta 2, Inuslin, IP-10, Leptin (OB), LIX, L-Selectin/CD62L, MCP-1, MDC, MIF, MIP-1 alpha, MIP-2, MIP-3 alpha, MMP-13, MMP-2, MMP-8, MuSK, Neuropilin-2, NGFR, Orexin A, Osteopontin/SPP1, PDGF-AA, Prolactin R, RAGE, RALT/MIG-6, RELM beta, Resistin, TL1A/TNFSF15/VEGI, TGF beta 1, TGF beta 2, TGF beta 3, Thrombospondin, TIE-2, TIMP-1, TIMP-2, TIMP-3, TLR4, TNF alpha, TRAIL, TROY, Ubiquitin+1, VEGF-A, VEGF-C
Rat
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Other Materials Required
Distilled or de-ionized water
KCl, NaCl, KH2PO4 and Na2HPO4
Small plastic or glass containers
Orbital shaker or oscillating rocker
Beaker, stir plate and stir bar
Pipettors, pipette tips and other common lab consumables
2~5 ml tube
50 ml conical collection tube
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
Dialyze samples in PBS
Biotinylate Samples
Dialyze biotinylated Samples in PBS
Block array membranes
Incubate with Samples
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.