Rat Immune Checkpoint Molecule Array 1

    


RayBio® C-Series Rat Immune Checkpoint Molecule Array 1 Kit. Detects 9 rat Immune Checkpoints. Suitable for all liquid sample types.



 AAR-ICM-1-2

Antigen Information

Gene Symbols:

  • CD27

  • CD80

  • CD86

  • FAS

  • FASLG

  • HAVCR1

  • LGALS1

  • LGALS3

  • TNFRSF9

Assay Format

Number of Targets Detected:
9
Species Detected:

  • Rat

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4 or 8 Sample Kit

Product Features

  • Easy to use

  • No specialized equipment needed

  • Compatible with nearly any liquid sample

  • Proven technology (many publications)

  • Highly sensitive (pg/ml)

  • Sandwich ELISA specificity

  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

4-1BB
(TNFRSF9/CD137)
CD27
(TNFRSF7)
CD80
(B7-1)
CD86
(B7-2)
Galectin-1
Galectin-3
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
TIM-1
(KIM-1)

Species Detected

Rat

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components

  • Rat Immune Checkpoint Array 1

  • Blocking Buffer

  • Wash Buffer 1

  • Wash Buffer 2

  • Biotinylated Detection Antibody Cocktail

  • Streptavidin-Conjugated HRP

  • Detection Buffer C

  • Detection Buffer D

  • Lysis Buffer

  • 8-Well Incubation Tray

  • Plastic Sheets

  • Array Templates

  • Manual

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Tissue Paper, blotting paper or chromatography paper

  • Adhesive tape or Saran Wrap

  • Distilled or de-ionized water

  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

  1. Block membranes

  2. Incubate with Sample

  3. Incubate with Biotinylated Detection Antibody Cocktail

  4. Incubate with HRP-Conjugated Streptavidin

  5. Incubate with Detection Buffers

  6. Image with chemiluminescent imaging system

  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.