Gallus (Chicken) Cytokine Array C1


RayBio® C-Series Gallus (Chicken) Cytokine Array C1 Kit. Detects 10 Gallus Cytokines. Suitable for all liquid sample types.


 AAG-CYT-1-2

Antigen Information

Accession Number:

  • F1P243

  • P26022

  • P49708

  • Q58IU6

  • Q6A2H4

  • Q6X0K9

  • Q8QG57

  • Q90923

  • Q90YI0

  • Q9PUK2

Gene Symbols:
CAR | CCL5 | IFNG | IL10 | IL12B | IL16 | IL21 | IL6 | NTN2 | NTN3   View more

Assay Format

Number of Targets Detected:
10
Species Detected:

  • Gallus (chicken)

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4 or 8 Sample Kit

Product Features

  • Easy to use

  • No specialized equipment needed

  • Compatible with nearly any liquid sample

  • Proven technology (many publications)

  • Highly sensitive (pg/ml)

  • Sandwich ELISA specificity

  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Caronte
IFN-gamma
IL-6
IL-10
IL-12 p40
IL-16
IL-21
Netrin-2
Pentraxin-3
(TSG-14)
RANTES
(CCL5)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components

  • Gallus (Chicken) Cytokine Antibody Array C1 Membranes

  • Blocking Buffer

  • Wash Buffer 1

  • Wash Buffer 2

  • Biotinylated Detection Antibody Cocktail

  • Streptavidin-Conjugated HRP

  • Detection Buffer C

  • Detection Buffer D

  • Lysis Buffer

  • 8-Well Incubation Tray

  • Plastic Sheets

  • Array Templates

  • Manual

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Tissue Paper, blotting paper or chromatography paper

  • Adhesive tape or Saran Wrap

  • Distilled or de-ionized water

  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

  1. Block membranes

  2. Incubate with Sample

  3. Incubate with Biotinylated Detection Antibody Cocktail

  4. Incubate with HRP-Conjugated Streptavidin

  5. Incubate with Detection Buffers

  6. Image with chemiluminescent imaging system

  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.