Rabbit Cytokine Array Q1

    


Quantibody® Rabbit (Lapine) Cytokine Array 1 Kit. Detects 10 Rabbit Cytokines. Suitable for all liquid sample types.



 QAL-CYT-1-1

Antigen Information

Gene Symbols:

  • CCL4

  • CXCL8

  • IL17A

  • IL1A

  • IL1B

  • IL21

  • LEP

  • MMP9

  • NCAM1

  • TNF

Assay Format

Pathway:

  • MAPK Signaling

  • NFkB Signaling

Number of Targets Detected:
10
Species Detected:

  • Rabbit

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Other Body Fluids

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA

  • High specificity and system reproducibility

  • Suitable for diverse sample types

  • Low sample volume requirement: 50 µL or less

  • Get results same day (6-hour processing time)

  • Well-suited for high throughput assays

  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-8
(CXCL8)
IL-17A
IL-21
Leptin
MIP-1 beta
(CCL4)
MMP-9
NCAM-1
(CD56)
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components

  • Rabbit Cytokine Array Q1 Slide(s)

  • Blocking Buffer

  • Wash Buffer 1

  • Wash Buffer 2

  • Lyophilized Standard Mix

  • Biotinylated Detection Antibody Cocktail

  • Streptavidin-Conjugated Fluor

  • Slide Washer/Dryer

  • Adhesive Plastic Strips

  • Manual

Other Materials Required

  • Distilled or deionized water

  • Small plastic boxes or containers

  • Pipettors, pipette tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Aluminum foil

  • Gene microarray scanner or similar laser fluorescence scanner


View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline

  1. Dry the glass slide

  2. Prepare Standards

  3. Block array surface

  4. Incubate with Samples and Standards

  5. Incubate with Biotinylated Detection Antibody Cocktail

  6. Incubate with Streptavidin-Conjugated Fluor

  7. Disassemble the glass slide

  8. Scan with a gene microarray laser scanner

  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.