Gene Symbols:CCL5
CXCL13
FSTL3
IL13
IL17F
IL1RN
KITLG
STAMBP
TNFSF12
VEGFA
Pathway:MAPK Signaling
NFkB Signaling
Number of Targets Detected:10
Method of Detection:Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Glass Slide
Size:16, 32, or 64 Sample Kit
RayBio
® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the
Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
High sensitivity (pg/mL to ng/mL levels)
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Test 16 samples on each slide
Data analysis software provides one-step computation
Scroll over each target protein for more information |
AMSH | BLC (CXCL13) | FLRG | IL-13 | IL-17F |
IL-1 Ra (IL-1 F3) | RANTES (CCL5) | SCF | TWEAK R (TNFRSF12) | VEGF-A |
Suggested Application
Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker Screening
Identifying Key Factors
Confirming a Biological Process
Other Materials Required
Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays!
Learn MoreProtocol Outline
Dry the glass slide
Prepare Standards
Block array surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.