Custom Human DCUN1D1 ELISA Custom Human DCUN1D1 ELISARayBio® Custom Human DCUN1D1 ELISA Kit. 5 - 7 week lead time.CODE: ELH-DCUN1D1-1Number of Plates: 1 Plate Kit 2 Plate Kit 5 Plate Kit Antigen InformationAccession Number:Q96GG9Protein Name & Synonyms:DCN1-like protein 1 (DCUN1 domain-containing protein 1) (Defective in cullin neddylation protein 1-like protein 1) (Squamous cell carcinoma-related oncogene)Assay FormatNumber of Targets Detected:1Species Detected:HumanDesign Principle:Sandwich-basedMethod of Detection:ColorimetricQuantitative/Semi-Quantitative:QuantitativeSolid Support:96-well MicroplateProduct SpecificationsSize:1, 2, or 5 x 96-Well Strip Microplate KitProduct FeaturesStrip plates and additional reagents allow for use in multiple experimentsQuantitative protein detectionEstablishes normal rangeThe best products for confirmation of antibody array dataApplication NotesKit ComponentsPre-Coated 96-well Strip MicroplateWash BufferStop SolutionAssay Diluent(s)Lyophilized StandardBiotinylated Detection AntibodyStreptavidin-Conjugated HRPTMB One-Step SubstrateOther Materials RequiredDistilled or deionized waterPrecision pipettes to deliver 2 µl to 1 µl volumesAdjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylindersTubes to prepare standard and sample dilutionsAbsorbent paperMicroplate reader capable of measuring absorbance at 450nmLog-log graph paper or computer and software for ELISA data analysisProtocol OutlinePrepare all reagents, samples and standards as instructed in the manual.Add 100 µl of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4°C.Add 100 µl of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 µl of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 µl of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 µl of Stop Solution to each well.Read at 450 nm immediately.Storage/StabilityThe entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.UsageThis product is furnished for LABORATORY RESEARCH USE ONLY.Not for diagnostic or therapeutic use. 最近产品Custom Human ZAP70 ELISAHuman ZAG ELISAHuman YY1 ELISA
