Human Cytokine Array Q1000

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Human Cytokine Array Q1000

Quantibody® Human Cytokine Array 1000 Kit. A combination of Human Inflammation Array Q3 and Human Growth Factor Array Q1. Detects 80 Human Inflammatory and Growth Factors. Suitable for all liquid sample types.

CODE: QAH-CAA-1000-1

Quantibody Sizes:

Antigen Information

Gene Symbols:

AREG | BDNF | BMP4 | BMP5 | BMP7 | CCL1 | CCL11 | CCL15 | CCL2 | CCL24 View more

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 100 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Amphiregulin	BDNF	beta-NGF	bFGF	BLC (CXCL13)
BMP-4	BMP-5	BMP-7	EGF	EGFR
Eotaxin-1 (CCL11)	Eotaxin-2 (MPIF-2/CCL24)	FGF-4	FGF-7 (KGF)	GCSF
GDF-15	GDNF	GM-CSF	Growth Hormone	EG-VEGF (PK1)
HB-EGF	HGF	I-309 (TCA-3/CCL1)	ICAM-1 (CD54)	IFN-gamma
IGF-1	IGFBP-1	IGFBP-2	IGFBP-3	IGFBP-4
IGFBP-6	IL-1 alpha (IL-1 F1)	IL-1 beta (IL-1 F2)	IL-1 ra (IL-1 F3)	IL-10
IL-11	IL-12 p40	IL-12 p70	IL-13	IL-15
IL-16	IL-17A	IL-2	IL-4	IL-5
IL-6	IL-6 R	IL-7	IL-8 (CXCL8)	Insulin
MCP-1 (CCL2)	M-CSF	M-CSF R	MIG (CXCL9)	MIP-1 alpha (CCL3)
MIP-1 beta (CCL4)	MIP-1 delta (CCL15)	NGFR (TNFRSF16)	NT-3	NT-4
Osteoprotegerin (TNFRSF11B)	PDGF-AA	PDGF-BB	PLGF	RANTES (CCL5)
SCF	SCF R (CD117/c-kit)	TGF alpha	TGF beta 1	TGF beta 3
TIMP-1	TIMP-2	TNF alpha	TNF beta (TNFSF1B)	TNF RI (TNFRSF1A)
TNF RII (TNFRSF1B)	VEGF-A	VEGF-D	VEGFR2	VEGFR3

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection
Biomarker Screening
Identifying Key Factors
Confirming Biological Process
Biomarker Validation
Validation of Antibody Array Results
Quantitative Protein Detection
Establishing Normal Range

Kit Components

Human Cytokine Array Q1000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.