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Human Nanog ELISA

首页 /产品中心/ 人ELISA Kit / Human Nanog ELISA

Human Nanog ELISA

RayBio® Human Nanog ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

CODE: ELH-NANOG-1

Number of Plates:

Antigen Information

Accession Number:

Q9H9S0

Gene ID:

79923

Gene Names:

NANOG

Protein Name & Synonyms:

Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog)

Assay Format

Detection Range:

0.34 ng/ml - 80 ng/ml

Recommended Dilution (serum & plasma):

2 fold

Sensitivity:

0.34 ng/ml

Specificity:

This ELISA antibody pair detects Human Nanog. Other species not determined.

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Plasma
Serum

Design Principle:

Sandwich-based

Method of Detection:

Colorimetric

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

96-well Microplate

Product Specifications

Size:

1, 2, or 5 x 96-Well Strip Microplate Kit

Standard Curves

Human Nanog ELISA(图1)

Spiking & Recovery Results

Human Nanog ELISA(图2)

Linearity Results

Human Nanog ELISA(图3)

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.